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python3-pychopper_2.7.2-1_all.deb
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مشاهده بیشترتوضیحات
identify, orient and trim full-length Nanopore cDNA reads
| ویژگی | مقدار |
|---|---|
| سیستم عامل | Linux |
| توزیع | Debian Bookworm-12 |
| مخزن | Debian main all |
| نام بسته | python3-pychopper |
| نام فایل بسته | python3-pychopper_2.7.2-1_all.deb |
| نسخه بسته | 2.7.2 |
| انتشار بسته | 1 |
| معماری بسته | all |
| نگهدارنده | Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org> |
| تاریخ ساخت | - |
| هاست سازنده | - |
| نوع بسته | .deb |
| آدرس صفحه اصلی | https://github.com/epi2me-labs/pychopper |
| مجوز | - |
| حجم دانلود | 136472 |
| حجم نصب | 407 |
Pychopper v2 is a Python module to identify, orient and trim full-length
Nanopore cDNA reads. It is also able to rescue fused reads and provides
the script 'cdna_classifier.py'. The general approach of Pychopper v2
is the following:
.
* Pychopper first identifies alignment hits of the primers across the
length of the sequence. The default method for doing this is using
nhmmscan with the pre-trained strand specific profile HMMs, included
with the package. Alternatively, one can use the edlib backend,
which uses a combination of global and local alignment to identify
the primers within the read.
* After identifying the primer hits by either of the backends, the
reads are divided into segments defined by two consecutive primer
hits. The score of a segment is its length if the configuration of
the flanking primer hits is valid (such as SPP,-VNP for forward reads)
or zero otherwise.
* The segments are assigned to rescued reads using a dynamic programming
algorithm maximizing the sum of used segment scores (hence the amount
of rescued bases). A crucial observation about the algorithm is that
if a segment is included as a rescued read, then the next segment
must be excluded as one of the primer hits defining it was "used
up" by the previous segment. This put constraints on the dynamic
programming graph. The arrows in read define the optimal path for
rescuing two fused reads with the a total score of l1 + l3.
.
A crucial parameter of Pychopper v2 is -q, which determines the
stringency of primer alignment (E-value in the case of the pHMM
backend). This can be explicitly specified by the user, however by
default it is optimized on a random sample of input reads to produce
the maximum number of classified reads.
نیازمندی
| مقدار | نام |
|---|---|
| - | python3-biopython |
| - | python3-matplotlib |
| - | python3-numpy |
| - | python3-pandas |
| - | python3-parasail |
| - | python3-pytest |
| - | python3-six |
| - | python3-tqdm |
| - | python3:any |
نحوه نصب
نصب پکیج deb python3-pychopper:
sudo apt-get install python3-pychopper_2.7.2-1_all.deb
فایل ها
| مسیرها |
|---|
| ./usr/bin/pychopper |
| ./usr/lib/python3/dist-packages/pychopper/__init__.py |
| ./usr/lib/python3/dist-packages/pychopper/alignment_hits.py |
| ./usr/lib/python3/dist-packages/pychopper/chopper.py |
| ./usr/lib/python3/dist-packages/pychopper/common_structures.py |
| ./usr/lib/python3/dist-packages/pychopper/edlib_backend.py |
| ./usr/lib/python3/dist-packages/pychopper/hmmer_backend.py |
| ./usr/lib/python3/dist-packages/pychopper/parasail_backend.py |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/PCS110_primers.hmm |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/PCS110_primers.hmm.h3f |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/PCS110_primers.hmm.h3i |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/PCS110_primers.hmm.h3m |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/PCS110_primers.hmm.h3p |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/__init__.py |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/cDNA_SSP_VNP.hmm |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/cDNA_SSP_VNP.hmm.h3f |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/cDNA_SSP_VNP.hmm.h3i |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/cDNA_SSP_VNP.hmm.h3m |
| ./usr/lib/python3/dist-packages/pychopper/phmm_data/cDNA_SSP_VNP.hmm.h3p |
| ./usr/lib/python3/dist-packages/pychopper/primer_data/PCS110_primers.fas |
| ./usr/lib/python3/dist-packages/pychopper/primer_data/PCS111_primers.fas |
| ./usr/lib/python3/dist-packages/pychopper/primer_data/__init__.py |
| ./usr/lib/python3/dist-packages/pychopper/primer_data/cDNA_SSP_VNP.fas |
| ./usr/lib/python3/dist-packages/pychopper/report.py |
| ./usr/lib/python3/dist-packages/pychopper/scripts/__init__.py |
| ./usr/lib/python3/dist-packages/pychopper/scripts/pychopper |
| ./usr/lib/python3/dist-packages/pychopper/scripts/pychopper.py |
| ./usr/lib/python3/dist-packages/pychopper/seq_utils.py |
| ./usr/lib/python3/dist-packages/pychopper/tests/__init__.py |
| ./usr/lib/python3/dist-packages/pychopper/tests/data/PCS111_umi_test_reads.fastq.gz |
| ./usr/lib/python3/dist-packages/pychopper/tests/data/PCS111_umi_test_reads_expected.fastq |
| ./usr/lib/python3/dist-packages/pychopper/tests/data/barcodes.fas |
| ./usr/lib/python3/dist-packages/pychopper/tests/data/expected_output.fas |
| ./usr/lib/python3/dist-packages/pychopper/tests/data/ref.fas.gz |
| ./usr/lib/python3/dist-packages/pychopper/tests/data/ref.fq.gz |
| ./usr/lib/python3/dist-packages/pychopper/tests/test_detector.py |
| ./usr/lib/python3/dist-packages/pychopper/tests/test_regression_simple.py |
| ./usr/lib/python3/dist-packages/pychopper/utils.py |
| ./usr/lib/python3/dist-packages/pychopper-2.7.2.egg-info/PKG-INFO |
| ./usr/lib/python3/dist-packages/pychopper-2.7.2.egg-info/dependency_links.txt |
| ./usr/lib/python3/dist-packages/pychopper-2.7.2.egg-info/entry_points.txt |
| ./usr/lib/python3/dist-packages/pychopper-2.7.2.egg-info/not-zip-safe |
| ./usr/lib/python3/dist-packages/pychopper-2.7.2.egg-info/requires.txt |
| ./usr/lib/python3/dist-packages/pychopper-2.7.2.egg-info/top_level.txt |
| ./usr/share/doc/python3-pychopper/changelog.Debian.gz |
| ./usr/share/doc/python3-pychopper/changelog.gz |
| ./usr/share/doc/python3-pychopper/copyright |
| ./usr/share/lintian/overrides/python3-pychopper |
| ./usr/share/man/man1/pychopper.1.gz |