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flybrains-0.2.7


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توضیحات

Transforms to map between different Drosophila template brains
ویژگی مقدار
سیستم عامل -
نام فایل flybrains-0.2.7
نام flybrains
نسخه کتابخانه 0.2.7
نگهدارنده []
ایمیل نگهدارنده []
نویسنده Philipp Schlegel
ایمیل نویسنده pms70@cam.ac.uk
آدرس صفحه اصلی https://github.com/schlegelp/navis-flybrains
آدرس اینترنتی https://pypi.org/project/flybrains/
مجوز GNU GPL V3
<p align="center"> <img src="https://github.com/schlegelp/navis-flybrains/blob/main/_static/flybrains_logo.png?raw=true" width="400"> </p> [![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.5515281.svg)](https://doi.org/10.5281/zenodo.5515281) # navis-flybrains Transforms to map between different _Drosophila_ template brains. Intended to be used with [navis](https://github.com/schlegelp/navis). This library is analogous to Greg Jefferis' [nat.templatebrains](https://github.com/natverse/nat.templatebrains), [nat.jrcbrains](https://github.com/natverse/nat.jrcbrains) and [nat.flybrains](https://github.com/natverse/nat.flybrains) for R. `flybrains` ships with: - meta data + surface meshes for 22 template brains and nerve cords - an Elastix transform between `FANC` and `JRCVNC2018F` kindly shared by Jasper Phelps - a landmark-based transform between `MANC` and `FANC` - mirror transforms for `FAFB14` and `FANC` There are plenty additional transforms that need to be downloaded separately (see below). ## Installation You can install `flybrains` from PyPI: ```bash pip3 install flybrains ``` ### External dependencies In order to use the Jefferis lab or VFB transforms, you will need to have [CMTK](https://www.nitrc.org/projects/cmtk/) installed. For the FANC to JRCVNC2018F transform, you will need to download [elastix](https://elastix.lumc.nl/index.php) and make sure that the path to the binaries is in your `PATH` variable. ## Usage <p align="center"> <img src="https://github.com/schlegelp/navis-flybrains/blob/main/_static/bridging_graph.png?raw=true" width="800"> </p> It's highly recommended that after install, you download the (optional) bridging transforms to map between template brains/nerve cords. **IMPORTANT**: the URL for the JRC2018F <-> JRC2018M transform (`JRC2018U_JRC2018M.h5`) was incorrect in `flybrains` version `0.2.6`. If you downloaded it using that version of flybrains you need to manually remove the file, update flybrains and download again using a newer version. > :exclamation: If you already have downloaded these registrations via `nat.jrcbrains` and/or `nat.flybrains` you can skip this: `flybrains` should be able to find the registrations downloaded via R and register them for you (see also code at the bottom). ```Python >>> import flybrains # This downloads (or updates) various CMTK bridging and mirror transforms # generated or collated by the Jefferis lab - see docstring for details >>> flybrains.download_jefferislab_transforms() # This downloads H5 bridging transforms between JRC brain templates # (Saalfeld lab, Janelia Research Campus) - see docstring for details >>> flybrains.download_jrc_transforms() # This downloads H5 bridging transforms between JRC VNC templates # (Saalfeld lab, Janelia Research Campus) - see docstring for details >>> flybrains.download_jrc_vnc_transforms() # This downloads (or updates) various CMTK bridging and mirror transforms # generated or collated by VirtualFlyBrain.org - see docstring for details >>> flybrains.download_vfb_transforms() # Register the transforms - this is only necessary if you just downloaded them # Alternatively just restart your Python session and import flybrains >>> flybrains.register_transforms() ``` In the future, simply importing `flybrains` is sufficient to make the transforms available to [navis](https://navis.readthedocs.io/en/latest/): ```Python >>> import navis >>> import flybrains >>> import numpy as np >>> points = np.array([[429536, 205240, 38400]]) >>> navis.xform_brain(points, source='FAFB', target='JRC2018F') array([[241.53969657, 100.99399233, 35.96977733]]) ``` Please see the [transform tutorial](https://navis.readthedocs.io/en/latest/source/tutorials/transforming.html) for `navis` to learn how to transform more complex data. To check which transforms are available (either downloaded or via R) you can run this: ```Python >>> # Generate a report - note the mix of transforms downloaded via Python and R >>> flybrains.report() Flybrains Status Report ======================= Data Home: /Users/philipps/flybrain-data CMTK registrations (Jefferis lab/VFB): 41 of 45 H5 registrations (JRC/Saalfeld lab): 3 of 8 nat regdirs ----------- ~/Library/Application Support/rpkg-nat.templatebrains/regfolders: 41 CMTK | 0 H5 transforms /Library/Frameworks/R.framework/Versions/3.6/Resources/library/nat.flybrains/extdata/bridgingregistrations: 5 CMTK | 0 H5 transforms /Library/Frameworks/R.framework/Versions/3.6/Resources/library/nat.flybrains/extdata/mirroringregistrations: 5 CMTK | 0 H5 transforms ~/Library/Application Support/R/nat.jrcbrains: 0 CMTK | 5 H5 transforms ``` Meta data and surface meshes for the template brains/VNCs are readily accessible: ```Python >>> flybrains.FAFB14 Template brain -------------- Name: Full Adult Fly Brain Short Name: FAFB14 Type: None Sex: female Dimensions: 165372 x 80745 x 6730 voxels Voxel size: x = 4 nanometers y = 4 nanometers z = 40 nanometers Bounding box (nanometers): x = 192200, y = 75853, z = 2007, x = 853686, y = 398832, z = 271205, Description: SSTEM volume comprising an entire female Drosophila brain was imaged at 4x4x40nm by Zheng et al. (2018) and is availabe for download at https://temca2data.org/. The meta data and associated mesh represent version 14 (FAFB14) of this data set. DOI: 10.1016/j.cell.2018.06.019 ``` Most templates come with a mesh e.g. for plotting via navis: ```Python >>> flybrains.FAFB14.mesh <trimesh.Trimesh(vertices.shape=(25047, 3), faces.shape=(50416, 3))> >>> # You can pass the template object directly to navis' plotting functions >>> navis.plot3d(flybrains.FAFB14) ``` ## Changes - `0.2.7` (05/01/23): fixed `JRC2018M` <-> `JRC2018U` transform download - ~~`0.2.6` (06/09/22): added `JRC2018M` <-> `JRC2018U` transform~~ (YANKED) - `0.2.5` (22/05/22): added `JRCFIB2022M` mesh and transform to/from `FAFB14` - `0.2.4` (12/05/22): added `FLYWIRE` template brain and landmark-based mirror transform - `0.2.0` (02/02/22): added VirtualFlyBrain.org's (CMTK) and Janelia's (H5) VNC transforms; renamed some download function - `0.1.14` (21/10/21): added `FANC` <-> `JRCVNC2018F` transform (requires Elastix and navis >=1.0.0) - `0.1.13` (14/10/21): add template and mirror transform for FANC - `0.1.12` (18/09/21): fixed directionality of Jefferis lab CMTK transforms - `0.1.11` (02/08/21): make downloads work if file size unknown - `0.1.10` (01/08/21): fix bug that led to warnings during transform registration on Windows systems - `0.1.9` (05/05/21): fixed mesh normals; fixed JRCFIB2018F units to nm and added JRCFIB2018Fum template; - `0.1.8` (10/04/21): add a simple symmetrization transform for FAFB: `FAFB14sym` - `0.1.7` (30/03/21): better deal with systems without nat libraries - `0.1.6` (25/03/21): fix bug that led to excessive recursive scanning of directories - `0.1.5` (03/03/21): fix bug that led to meshes not being packaged - `0.1.4` (24/02/21): added "hemibrain" alias for "JRCFIB2018F"; added hemibrain bounding box mesh - `0.1.3` (12/01/21): improved the warp mirror registration for `FAFB14` - `0.1.2` (10/01/21): added a warp mirror registration for `FAFB14` - `0.1.1` (06/01/21): added `um` (for microns) suffix to `JRCFIB2018F` transforms; added affine `JRCFIB2018Fraw` -> `JRCFIB2018F` -> `JRCFIB2018Fum` transforms - `0.1.0` (03/01/21): first working version ## Acknowledgements Critically based on `nat.flybrains` and `nat.jrcbrains` by Greg Jefferis _et al._ for inspiration for the implementation and meta data on e.g. template brains. As reference for the Jefferis lab registrations, please use: ``` The natverse, a versatile toolbox for combining and analysing neuroanatomical data. A.S. Bates, J.D. Manton, S.R. Jagannathan, M. Costa, P. Schlegel, T. Rohlfing, G.S. Jefferis eLife. 9 (2020) e53350. doi:10.7554/eLife.53350. ``` As (partial) reference for the Saalfeld lab registrations, please see: ``` An unbiased template of the Drosophila brain and ventral nerve cord. John A Bogovic, Hideo Otsuna, Larissa Heinrich, Masayoshi Ito, Jennifer Jeter, Geoffrey Meissner, Aljoscha Nern, Jennifer Colonell, Oz Malkesman, Kei Ito, Stephan Saalfeld PLOS One; doi: https://doi.org/10.1371/journal.pone.0236495 ``` For references of individual template brains, please see their docstrings: ```Python >>> help(flybrains.IBN) ```


نیازمندی

مقدار نام
- gitpython
>=0.6.0 navis
- numpy
- pandas


زبان مورد نیاز

مقدار نام
>=3.6 Python


نحوه نصب


نصب پکیج whl flybrains-0.2.7:

    pip install flybrains-0.2.7.whl


نصب پکیج tar.gz flybrains-0.2.7:

    pip install flybrains-0.2.7.tar.gz