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cytopast-1.1.1


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توضیحات

Visualisation of PastML trees.
ویژگی مقدار
سیستم عامل -
نام فایل cytopast-1.1.1
نام cytopast
نسخه کتابخانه 1.1.1
نگهدارنده []
ایمیل نگهدارنده []
نویسنده Anna Zhukova
ایمیل نویسنده anna.zhukova@pasteur.fr
آدرس صفحه اصلی https://github.com/evolbioinfo/cytopast
آدرس اینترنتی https://pypi.org/project/cytopast/
مجوز -
# cytopast __cytopast__ is a python3 module that creates zoomable HTML visualisation of phylogenetic trees with annotated nodes. Given a tree and its node annotations, it can either visualise them as-is, or apply [PastML](https://github.com/saishikawa/PASTML) to infer ancestral states based on the tip states. The states are visualised as different colours of the tree nodes using [Cytoscape.js](http://js.cytoscape.org/) # Article For a detailed description of PastML/cytopast: see Ishikawa SA, Zhukova A, Iwasaki W, Gascuel O (2018) __A Fast Likelihood Method to Reconstruct and Visualize Ancestral Scenarios__ [[bioRxiv]](https://doi.org/10.1101/379529). # Input data As an input, one needs to provide a **rooted** phylogenetical tree in [newick](https://en.wikipedia.org/wiki/Newick_format) format, and a table containing tip states, in tab-delimited (by default) or csv format (to be specified with *--data_sep ,* option). ### Example You can download [HIV1-A in Albania data](examples/Albania/data) as an example. Let's assume that the tree and annotation files are in the Downloads folder, and are named respectively Albanian.tree.152tax.tre and data.txt. The data.txt is a comma-separated file, containing tip ids in the first column, and Country in the second column, i.e.: id | Country ----- | ----- 98CMAJ6932 | Africa 98CMAJ6933 | Africa 96CMAJ6134 | Africa 00SEAY5240 | WestEurope ... | ... 02GRAY0303 | Greece 97YUAF9960 | EastEurope # Try it online Try it at [pastml.pasteur.fr](https://pastml.pasteur.fr) # Run it on your computer There are 2 alternative ways to run cytopast on your computer: with [docker](https://www.docker.com/community-edition), or in python3. ## Run with docker ### Basic usage Once [docker](https://www.docker.com/community-edition) is installed, run the following command: ```bash docker run -v <path_to_the_folder_containing_the_tree_and_the_annotations>:/data:rw -t evolbioinfo/pastml --tree /data/<tree_file> --data /data/<annotation_file> --data_sep <separator_eg_comma> --columns <one_or_more_column_names> --html_compressed /data/<map_name> ``` For example, to reconstruct and visualise the ancestral Country states for Albanian data, one needs to run the following command: ```bash docker run -v ~/Downloads:/data:rw -t evolbioinfo/pastml --tree /data/Albanian.tree.152tax.tre --data /data/data.txt --data_sep , --columns Country --html_compressed /data/Albanian_map.html ``` This will produce a file Albanian_map.html in the Downloads folder, that can be viewed with a browser. ### Help To see advanced options, run ```bash docker run -t evolbioinfo/pastml -h ``` ## Run in python3 ### Windows For **Windows** users, we recommend installing cytopast via [Cygwin environment](https://www.cygwin.com/). First instal gcc, gsl, python3 and pip3 from the Cygwin packages. Then install cytopast: ```bash pip3 install cytopast ``` ### All other platforms We strongly recommend installing cytopast for python via [conda](https://conda.io/docs/), following the procedure described below: #### Installing with conda Once you have conda installed create an environment for cytopast with python3, gcc and gsl: ```bash conda create --name cytopast python=3 gcc gsl ``` Then activate it: ```bash source activate cytopast ``` Then install cytopast in it: ```bash pip install cytopast ``` #### Installing without conda Install [GNU GSL](https://www.gnu.org/software/gsl/), following the instructions provided on GSL website. Then install cytopast: ```bash pip3 install cytopast ``` ### Basic usage in a command line If you installed cytopast via conda, do not forget to first activate the dedicated environment, e.g. ```bash source activate cytopast ``` To run cytopast: ```bash cytopast --tree <path/to/tree_file.nwk> --data <path/to/annotation_file.tab> --columns <one_or_more_column_names> --html_compressed <path/to/output/map.html> --data_sep <separator_eg_comma> ``` For example, to reconstruct and visualise the ancestral Country states for Albanian data, one needs to run the following command: ```bash cytopast --tree ~/Downloads/Albanian.tree.152tax.tre --data ~/Downloads/data.txt --data_sep , --columns Country --html_compressed ~/Downloads/Albanian_map.html ``` This will produce a file Albanian_map.html in the Downloads folder, that can be viewed with a browser. ### Help To see advanced options, run: ```bash cytopast -h ``` ### Basic usage in python3 ```python from cytopast.pastml_analyser import pastml_pipeline # Path to the table containing tip/node annotations, in csv or tab format data = "~/Downloads/data.txt" # Path to the tree in newick format tree = "~/Downloads/Albanian.tree.152tax.tre" # Columns present in the annotation table, # for which we want to reconstruct ancestral states # (for Albanian data we only have one column, but multiple columns are also allowed) columns = ['Country'] # Path to the output compressed map visualisation html_compressed = "~/Downloads/Albanian_map.html" # (Optional) path to the output tree visualisation html = "~/Downloads/Albanian_tree.html" pastml_pipeline(data=data, data_sep=',', columns=columns, name_column='Country', tree=tree, html_compressed=html_compressed, html=html, verbose=True) ``` ### Examples See the [examples folder](https://github.com/evolbioinfo/cytopast/tree/master/examples) for ideas :)


نحوه نصب


نصب پکیج whl cytopast-1.1.1:

    pip install cytopast-1.1.1.whl


نصب پکیج tar.gz cytopast-1.1.1:

    pip install cytopast-1.1.1.tar.gz