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cloning-0.0.0
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مشاهده بیشترتوضیحات
Simulate DNA cloning reactions.
| ویژگی | مقدار |
|---|---|
| سیستم عامل | - |
| نام فایل | cloning-0.0.0 |
| نام | cloning |
| نسخه کتابخانه | 0.0.0 |
| نگهدارنده | [] |
| ایمیل نگهدارنده | [] |
| نویسنده | - |
| ایمیل نویسنده | Kale Kundert <kale@thekunderts.net> |
| آدرس صفحه اصلی | - |
| آدرس اینترنتی | https://pypi.org/project/cloning/ |
| مجوز | - |
*******
Cloning
*******
.. image:: https://img.shields.io/pypi/v/cloning.svg
:alt: Last release
:target: https://pypi.python.org/pypi/cloning
.. image:: https://img.shields.io/pypi/pyversions/cloning.svg
:alt: Python version
:target: https://pypi.python.org/pypi/cloning
.. image:: https://img.shields.io/readthedocs/cloning.svg
:alt: Documentation
:target: https://cloning.readthedocs.io/en/latest/?badge=latest
.. image:: https://img.shields.io/github/workflow/status/kalekundert/cloning/Test%20and%20release/master
:alt: Test status
:target: https://github.com/kalekundert/cloning/actions
.. image:: https://img.shields.io/coveralls/kalekundert/cloning.svg
:alt: Test coverage
:target: https://coveralls.io/github/kalekundert/cloning?branch=master
.. image:: https://img.shields.io/github/last-commit/kalekundert/cloning?logo=github
:alt: Last commit
:target: https://github.com/kalekundert/cloning
The purpose of this project is to provide the ability to simulate any step
involved in cloning DNA constructs, e.g. PCR, restriction digests, Gibson
assemblies, Golden Gate assemblies, etc. Some implementation details:
- DNA is represented with a very high level of detail and generality, so that
things like sticky ends, phosphorylated ends, modified nucleotides etc. can
be accounted for. For example, something like this:
.. code-block:: python
class Duplex:
watson: Strand
crick: Strand
class Strand:
# Generally the graph will be a simple linked list, but it is possible
# for nucleotides to branch.
polymer: nx.Graph
class Nucleotide:
# There's a library called `pysmiles` than can construct graphs of
# atoms from SMILES strings.
atoms: nx.Graph
# Node indices in the above graph.
attachment_points: int
# e.g. ATCG
symbol: str
class DegenerateNucleotide:
mix: Dict[Nucleotide, float]
def dsdna_from_str(str, *, phos_5, phos_3, sticky_5, sticky_3):
# I'll want a lot of keyword argument to control how the duplex is
# constructed. I've listed a few here, but I haven't really thought
# about the format they'd take.
pass
def ssdna_from_str(...):
pass
def dsrna_from_str(...):
pass
def ssrna_from_str(...):
pass
At the same time, every effort is made to accept simple strings wherever a
sequence is required, for convenience.
- Cloning steps would be implemented as simple functions, for the most part.
Maybe in some cases it'd be better to use functors, just to make it easier to
pass lots of parameters, but I don't want to lean into that. Some examples:
.. code-block:: python
def pcr(template, primer1, primer_2):
# Check that primers face each other (accounting for circular
# templates) and that product is unambiguous.
return Duplex(...)
def gibson(fragments):
# - Build graph using ends of each fragment
# - Make sure that the graph is circular, and uses each fragment
# exactly once.
return Duplex(..., circular=True)
def golden_gate(fragments):
# Similar to Gibson.
return Duplex(..., circular=True)
def digest(duplex, enzymes):
return Duplex(...)
def phosphorylate(strand):
# If duplex provided, phosphorylate both strands.
return Strand(...)
def ligate(fragments):
# - Require phosphorylated ends.
# - Require a single unique product, by default.
return Duplex(..., circular=True)
def anneal(oligo_1, oligo_2):
return Duplex(...)
def transcribe(template):
# Check for promoter. Maybe optionally require GGG for T7.
return Strand(..., rna=True)
def express(template, start_codon=0):
# - Require RNA template
# - Third party functions should be used to predict start codon from
# transcript, if the user needs that necessary.
return Strand()
- Some general-purpose tools that I'd like to include:
- Reverse complement.
- Translation.
- Melting temperature calculation (via Biopython).
- Sequence alignment, especially for circular sequences.
- Support for parsing IDT sequence strings.
- Some general purpose tools I'm hesitant to include:
- Reverse translation: Doing this for any real application is a pretty
intense optimization problem, e.g. finding a sequence that uses common
codons, avoids restriction sites, minimizes internal
RBSs/promoters/terminators, isn't too complex, etc. I think this should be
the domain of a devoted tool.
Some ideas about names:
- `cloning`: Shocking that this is available.
- `biopolymers`: Might be a better fit for the actual function, since it makes
sense to include `transcribe()` and `express()` functions.
- `biopol`/`biopols`: Abbreviations of above.
نیازمندی
| مقدار | نام |
|---|---|
| - | sphinx |
| - | sphinx_rtd_theme |
| - | autoclasstoc |
| - | pytest |
| - | pytest-cov |
| - | parametrize_from_file |
| - | coveralls |
زبان مورد نیاز
| مقدار | نام |
|---|---|
| ~=3.7 | Python |
نحوه نصب
نصب پکیج whl cloning-0.0.0:
pip install cloning-0.0.0.whl
نصب پکیج tar.gz cloning-0.0.0:
pip install cloning-0.0.0.tar.gz