# bold_identification
## 1 Introduction
see `https://github.com/linzhi2013/bold_identification`.
This is a Python3 package which can get the taxonomy information of sequences from BOLD [http://www.boldsystems.org/index.php](http://www.boldsystems.org/index.php).
Currently, `bold_identification` only runs on Mac OS, Windows 64bit, Linux.
## 2 Installation
[](http://bioconda.github.io/recipes/bold-identification/README.html)
Or with `pip`
$ pip install bold_identification
There will be a command `bold_identification` created under the same directory as your `pip` command.
## 3 Usage
run `bold_identification`
$ bold_identification
usage: bold_identification [-h] -i <str> [-f <str>] -o <str>
[-d {COX1,COX1_SPECIES,COX1_SPECIES_PUBLIC,COX1_L640bp,ITS,MATK_RBCL}] [-n <int>] [-r <int>]
[-c] [-C] [-q <int>] [-D] [--version]
To identify taxa of given sequences from BOLD (http://www.boldsystems.org/).
Some sequences can fail to get taxon information, which can be caused by
TimeoutException if your network to the BOLD server is bad.
Those sequences will be output in the file '*.TimeoutException.fasta'.
You can:
1) run another searching with the same command directly (but add -c option);
2) lengthen the time to wait for each query (-t option);
3) increase submission times (-r option) for a sequence.
Also, the sequences without BOLD matches will be output in the
file '*.NoBoldMatchError.fasta'
By Guanliang Meng.
See https://github.com/linzhi2013/bold_identification.
Citation:
Yang C, Zheng Y, Tan S, Meng G, et al.
Efficient COI barcoding using high throughput single-end 400 bp sequencing.
https://doi.org/10.1186/s12864-020-07255-w
version: 0.0.27
optional arguments:
-h, --help show this help message and exit
-i <str> input file name
-f <str> input file format [fasta]
-o <str> outfile prefix
-d {COX1,COX1_SPECIES,COX1_SPECIES_PUBLIC,COX1_L640bp,ITS,MATK_RBCL}
database to search [COX1]
-n <int> how many first top hits will be output. [1]
-r <int> Maximum submission time for a sequence, useful for handling TimeOutException. [4]
-c continuous mode, jump over the ones already in "-o" file, will resubmit all the remained. use "-cc"
to also jump over the ones in "*.NoBoldMatchError.fasta" file.
-C For chimera check purpose. If set, for each sequence, I will query the BOLD database using the
subsequences from 5'- and 3'-ends with a length of '-q <int>' bp, respectively
-q <int> The length of subsequences for chimera check [400]
-D debug mode output [False]
--version show program's version number and exit
## 4 Citation
When you use `bold_identification` in your study, please cite:
Yang C, Zheng Y, Tan S, Meng G, et al.
Efficient COI barcoding using high throughput single-end 400 bp sequencing.
https://doi.org/10.1186/s12864-020-07255-w
