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ResPAN-0.1.0
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مشاهده بیشترتوضیحات
A light structured residual autoencoder and mutual nearest neighbor paring guided adversarial network for scRNA-seq batch correction.
| ویژگی | مقدار |
|---|---|
| سیستم عامل | OS Independent |
| نام فایل | ResPAN-0.1.0 |
| نام | ResPAN |
| نسخه کتابخانه | 0.1.0 |
| نگهدارنده | [] |
| ایمیل نگهدارنده | [] |
| نویسنده | Yuge Wang, Tianyu Liu |
| ایمیل نویسنده | wangyuge22@qq.com |
| آدرس صفحه اصلی | https://github.com/AprilYuge/ResPAN |
| آدرس اینترنتی | https://pypi.org/project/ResPAN/ |
| مجوز | MIT License |
# ResPAN
This reporsity contains code and information of data used in the paper “*ResPAN: a powerful batch correction model for scRNA-seq data through residual adversarial networks*”. Source code for ResPAN are in the [ResPAN](https://github.com/AprilYuge/ResPAN/tree/main/ResPAN) folder, scipts for reproducing benchmarking results are in the [scripts](https://github.com/AprilYuge/ResPAN/tree/main/scripts) folder, and data information can be found in the [data](https://github.com/AprilYuge/ResPAN/tree/main/data) folder.
ResPAN is a light structured **Res**idual autoencoder and mutual nearest neighbor **P**aring guided **A**dversarial **N**etwork for scRNA-seq batch correction. The workflow of ResPAN contains three key steps: generation of training data, adversarial training of the neural network, and generation of corrected data without batch effect. A figure summary is shown below.
.
More details about ResPAN can be found in our [manuscript](https://www.biorxiv.org/content/10.1101/2021.11.08.467781v3.full).
### Package requirement
ResPAN is implemented in Python and based on the framework of PyTorch. Before downloading and installing ResPAN, some packages need to be installed first. These required packages along with their versions used in our manuscript are listed below.
| Package | Version |
|------------|--------------|
| numpy | 1.18.1 |
| pandas | 1.3.5 |
| scipy | 1.8.0 |
| scanpy | 1.8.2 |
| pytorch | 1.10.2+cu113 |
### Download
To download and install ResPAN, please copy and paste the following line to your terminal:
```
git clone https://github.com/AprilYuge/ResPAN.git
```
### Brief tutorial
A brief tutorial of using ResPAN can be found below and under the folder [tutorials](https://github.com/AprilYuge/ResPAN/tree/main/tutorials).
To run our method, the first thing is to import necessary packages:
```
import numpy as np
import pandas as pd
import scanpy as sc
import scipy
from ResPAN import run_respan
```
Then we need to load the scRNA-seq data with batch information and preprocess it before running ResPAN:
```
# data loading
adata = sc.read_loom('CL_raw.loom', sparse=False)
# pre-processing
sc.pp.filter_cells(adata, min_genes=200)
sc.pp.filter_genes(adata, min_cells=3)
sc.pp.normalize_per_cell(adata, counts_per_cell_after=1e4)
sc.pp.log1p(adata)
sc.pp.highly_variable_genes(adata, n_top_genes=2000, batch_key='batch')
adata = adata[:, adata.var['highly_variable']]
# check if data is in sparse format
if isinstance(adata.X, scipy.sparse.csr.csr_matrix):
adata_new = sc.AnnData(adata.X.todense())
adata_new.obs = adata.obs.copy()
adata_new.obs_names = adata.obs_names
adata_new.var_names = adata.var_names
adata_new.obs_names.name = 'CellID'
adata_new.var_names.name = 'Gene'
del adata
adata = adata_new
```
Now we can run ResPAN on the preprocessed data for batch correction. The output result is an AnnData object:
```
adata_new = run_respan(adata, batch_key='batch', epoch=300, batch=1024, reduction='pca', subsample=3000, seed=999)
```
As indicated in our manuscipt, we use PCA for dimensionality reduction, kPCA (`reduction='kpca'`) and CCA (`reduction='cca'`) are also implemented, but their performance were not as good as PCA. Meanwhile, we subsampled cells in each batch to 3,000 before finding random walk MNN pairs [1].
To visualize our results, we can use the following commands:
```
adata_new.raw = adata_new
sc.pp.scale(adata_new, max_value=10)
sc.tl.pca(adata_new, 20, svd_solver='arpack')
sc.pp.neighbors(adata_new)
sc.tl.umap(adata_new)
sc.set_figure_params(figsize=(5,5),fontsize=12)
sc.pl.umap(adata_new, color=['batch', 'celltype'], frameon=False, show=False)
```
### Code references
For the implementation of ResPAN, we referred to [WGAN-GP](https://github.com/Zeleni9/pytorch-wgan) for the structure of Generative Adversarial Network and [iMAP](https://github.com/Svvord/iMAP) for the random walk mutual nearest neighbor method. Many thanks to their open-source treasure.
### Paper references
[1] Wang, Dongfang, et al. "iMAP: integration of multiple single-cell datasets by adversarial paired transfer networks." Genome biology 22.1 (2021): 1-24.
نیازمندی
| مقدار | نام |
|---|---|
| - | numpy |
| - | pandas |
| - | scipy |
| - | torch |
| - | sklearn |
| - | scanpy |
نحوه نصب
نصب پکیج whl ResPAN-0.1.0:
pip install ResPAN-0.1.0.whl
نصب پکیج tar.gz ResPAN-0.1.0:
pip install ResPAN-0.1.0.tar.gz