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توضیحات

A package for aligning RNA-seq data without reference biases

ویژگی	مقدار
سیستم عامل	OS Independent
نام فایل	RNA-APoGee-0.0.9
نام	RNA-APoGee
نسخه کتابخانه	0.0.9
نگهدارنده	[]
ایمیل نگهدارنده	[]
نویسنده	Sofia Panagiotopoulou
ایمیل نویسنده	spanagiotopoulou@gillumina.com
آدرس صفحه اصلی	https://git.illumina.com/spanag/APoGee
آدرس اینترنتی	https://pypi.org/project/RNA-APoGee/
مجوز	-

# RNA-APoGee RNA-APoGee (RNA Alignment to Personal Genomes) is a package to align RNA-seq data while minimizing reference biases. It can also be used to align RNA-seq data to haplotype resolved variants. Currently, RNA-APoGee relies on the Olego aligner, although other aligners could be used instead. ## Pre-requisites: - RNA-APoGee has only been tested on Linux and requires Python 3. - [Olego](https://zhanglab.c2b2.columbia.edu/index.php/OLego_Documentation) must be installed and on your PATH. - [samtools](http://samtools.sourceforge.net/) ## Installation ```pip install RNA-APoGee``` ## Command line utilities Alignment involves two steps: 1. Generating a "personalized" genome that has the variants of the individual embedded into the reference genome. 2. Aligning against the reference and the personal genome (or against two haplotypes) and then merging the two sets of alignment to pick the best alignment for each read. ### Generating a personal genome `create_genomes` creates versions of an input FASTA with sample-specific SNVs replacing reference bases. If you have phased variants, you can create two VCFs corresponding to the variants of each haplotype and then create two versions of the reference by calling `create_genomes` twice, once for each haplotype (unfortunately currently this script ignores the phasing of the variants.) ``` create_genomes --fasta FASTA --vcf VCF --outdir OUTDIR [--samples SAMPLES] [--min_gq MIN_GQ] [--chunk CHUNK] --fasta FASTA FASTA file that will be used as the base for generating personal genomes. For each sample in the input VCF, an individual genome will be created by substituting the sample's SNVs into this base FASTA. SNVs will be considered only if the FILTER field is PASS, and the genotype quality is greater than <min_gq>. --vcf VCF VCF with variant calls. Can have multiple samples. --outdir OUTDIR Personal genome for sample <sample> will be in <outdir>/<sample>.fa --samples SAMPLES (Optional) Comma separated list of samples from the input VCF. If provided, only the personal genomes for these samples will be created, otherwise personal genomes for all samples in the input VCF will be created. --min_gq MIN_GQ (Optional) Minimum genotype quality to consider a variant --chunk CHUNK (Optional) How many bases to keep in memory. Reduce if running OOM. ``` ### Aligning against the reference and the personal genome `apogee` aligns RNA-seq data to a personalized genome. Each read (or read-pair in case of paired data) is aligned against two FASTAs (correponding to two haplotypes or to a reference with and without an individual's variants). Then for each read (or read-pair) the best alignment across the two FASTAs is chosen. The order in which the two references are given (i.e. which one is specified as `ref_fasta` and which one is specified as `alt_fasta`) does not matter. Note that a lot of intermediate files are created. If `tmp_dir` is specified, all intermediate files will be stored there, with a prefix matching the prefix of the output BAM. In this case, it's up to you to delete that directory. If `tmp_dir` is not specified a temporary directory will be created, in the same directory as the output BAM and then deleted (so all intermediate files will be lost). ``` apogee --fq1 FQ1 --ref_fasta REF_FASTA --alt_fasta ALT_FASTA --bam BAM [--fq2 FQ2] [--tmp_dir TMP_DIR] --fq1 FQ1 FASTQ file with all reads (for single-end) or read1 reads (for paired-end) --fq2 FQ2 (Optional) FASTQ file with read2 reads --ref_fasta REF_FASTA First FASTA against which to align --alt_fasta ALT_FASTA Second FASTA against which to align --bam BAM Output BAM --tmp_dir TMP_DIR (Optional) Directory of intermediate files --threads THREADS (Optional) Number of threads for alignment [1] ```

نیازمندی

مقدار	نام
>=1.16.4	numpy
>=0.25.1	pandas
>=0.5.2	pyfasta
>=0.15.2	pysam
>=0.6.8	PyVCF

زبان مورد نیاز

مقدار	نام
>=3.6	Python

نحوه نصب

نصب پکیج whl RNA-APoGee-0.0.9:

pip install RNA-APoGee-0.0.9.whl

نصب پکیج tar.gz RNA-APoGee-0.0.9:

pip install RNA-APoGee-0.0.9.tar.gz