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ACTIONet-0.3.0
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مشاهده بیشترتوضیحات
ACTIONet single-cell analysis framework
| ویژگی | مقدار |
|---|---|
| سیستم عامل | - |
| نام فایل | ACTIONet-0.3.0 |
| نام | ACTIONet |
| نسخه کتابخانه | 0.3.0 |
| نگهدارنده | [] |
| ایمیل نگهدارنده | [] |
| نویسنده | Shahin Mohammadi |
| ایمیل نویسنده | shahin.mohammadi@gmail.com |
| آدرس صفحه اصلی | https://github.com/shmohammadi86/ACTIONet |
| آدرس اینترنتی | https://pypi.org/project/ACTIONet/ |
| مجوز | - |
# Installation
### Setting Up the Environment (Preinstallation)
**For Linux Users**
Verify that the cmake version you are using is >=3.19.
For the optimal performance on Intel-based architectures, installing [Intel Math Kernel Library (MKL)](https://software.intel.com/content/www/us/en/develop/articles/intel-math-kernel-library-intel-mkl-2020-install-guide.html) is **highly** recommended. After installing, make sure `MKLROOT` is defined by running the [setvars](https://software.intel.com/content/www/us/en/develop/documentation/using-configuration-file-for-setvars-sh/top.html) script.
**Install library dependencies**
To install the `ACTIONet` dependencie on debian-based linux machines, run:
```bash
sudo apt-get install libhdf5-dev libsuitesparse-dev libnss3 xvfb libblas-dev liblapack-dev
```
For Mac-based systems, you can use [brew](https://brew.sh/) instead:
```bash
brew install hdf5 suite-sparse c-blosc blas lapack
```
### Installing ACTIONet Python Package
Use `pip` to install ACTIONet directly from this repository:
```bash
pip install git+https://github.com/shmohammadi86/ACTIONet@python-devel
```
To install from source:
```
git clone --recurse-submodules https://github.com/shmohammadi86/ACTIONet.git
make install
```
# Running ACTIONet
**Note** If you are using `MKL`, make sure to properly [set the number of threads](https://software.intel.com/content/www/us/en/develop/documentation/mkl-macos-developer-guide/top/managing-performance-and-memory/improving-performance-with-threading/techniques-to-set-the-number-of-threads.html) used prior to running `ACTIONet`.
## Example Run
Here is a simple example to get you started:
```python
import urllib.request
import ACTIONet as an
import scanpy as sc
# Download example dataset from the 10X Genomics website
opener = urllib.request.build_opener()
opener.addheaders = [('User-agent', 'Mozilla/5.0')]
urllib.request.install_opener(opener)
urllib.request.urlretrieve('http://cf.10xgenomics.com/samples/cell-exp/3.0.0/pbmc_10k_v3/pbmc_10k_v3_filtered_feature_bc_matrix.h5', 'pbmc_10k_v3.h5')
# Read and filter the data
adata = sc.read_10x_h5('pbmc_10k_v3.h5')
adata.var_names_make_unique(join='.')
an.pp.filter_adata(adata, min_cells_per_feature=0.01, min_features_per_cell=1000)
sc.pp.normalize_total(adata)
sc.pp.log1p(adata)
# Run ACTIONet
an.pp.reduce_kernel(adata)
an.run_ACTIONet(adata)
# Annotate cell-types
marker_genes, directions, names = an.tl.load_markers('PBMC_Monaco2019_12celltypes')
cell_labels, confidences, Z = an.tl.annotate_cells_using_markers(adata, marker_genes, directions, names)
adata.obs['celltypes'] = cell_labels
# Visualize output
an.pl.plot_ACTIONet(adata, 'celltypes', transparency_key='node_centrality')
# Export results
adata.write('pbmc_10k_v3.h5ad')
```
## Visualizing results using cellxgene
The output of ACTIONet in the python implementation is internally stored as as `AnnData` object, and R `ACE` objects can be imported from/exported to `AnnData` using functions `AnnData2ACE()` and `ACE2AnnData()` functions, respectively. `AnnData` objects can be directly loaded into [cellxgene](https://github.com/chanzuckerberg/cellxgene) package, an open-source viewer for interactive single-cell data visualization. `cellxgene` can be installed as:
```bash
pip install cellxgene
```
Then to visualize the results of ACTIONet, run:
```bash
cellxgene launch pbmc_10k_v3.h5ad
```
where *pbmc_10k_v3.h5ad* is the name of the file we exported using `adata.write()` function.
# Additional tutorials
You can access ACTIONet tutorials from:
1. [ACTIONet framework at a glance (human PBMC 3k dataset)](http://compbio.mit.edu/ACTIONet/tutorials/mini_intro.html)
2. [Introduction to the ACTIONet framework (human PBMC Granja et al. dataset)](http://compbio.mit.edu/ACTIONet/tutorials/intro.html)
3. [Introduction to cluster-centric analysis using the ACTIONet framework](http://compbio.mit.edu/ACTIONet/tutorials/clustering.html)
4. [To batch correct or not to batch correct, that is the question!](http://compbio.mit.edu/ACTIONet/tutorials/batch.html)
5. [PortingData: Import/export options in the ACTIONet framework](http://compbio.mit.edu/ACTIONet/tutorials/porting_data.html)
6. [Interactive visualization, annotation, and exploration](http://compbio.mit.edu/ACTIONet/tutorials/annotation.html)
7. [Constructing cell-type/cell-state-specific networks using SCINET](http://compbio.mit.edu/ACTIONet/tutorials/scinet.html)
You can also find a [step-by-step guide](http://compbio.mit.edu/ACTIONet/tutorials/guide.html) to learning the core functionalities of the ACTIONet framework.
نیازمندی
| مقدار | نام |
|---|---|
| - | cmake |
| ==0.8.0 | anndata |
| ==1.9.1 | scanpy |
| >=3.0 | h5py |
| >=0.7.3 | adjustText |
| >=7.0.1 | natsort |
| >=1.19.2 | numpy |
| >=1.1.5 | pandas |
| >=1.5.2 | scipy |
| >=3.7.4 | typing-extensions |
| - | plotly |
| - | seaborn |
| - | orca |
| - | sphinx |
| - | nbsphinx |
| - | mypy |
| - | harmonypy |
زبان مورد نیاز
| مقدار | نام |
|---|---|
| >=3.8 | Python |
نحوه نصب
نصب پکیج whl ACTIONet-0.3.0:
pip install ACTIONet-0.3.0.whl
نصب پکیج tar.gz ACTIONet-0.3.0:
pip install ACTIONet-0.3.0.tar.gz